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    Nikon algorithms fiji imagej nih
    Algorithms Fiji Imagej Nih, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/algorithms+fiji+imagej+nih/NIS-Elements/pm41861814-221-189-197
    Average 99 stars, based on 39798 article reviews
    algorithms fiji imagej nih - by Bioz Stars, 2026-09
    99/100 stars

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    Protease Inhibitor:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Recombinant:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Article Title: Shed CNTNAP2 ectodomain is detectable in CSF and regulates Ca 2+ homeostasis and network synchrony via PMCA2/ATP2B2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CNTNAP2 antibody (C-term) Millipore Cat# AB5886, RRID: AB_92118 Mouse monoclonal anti-CNTNAP2 antibody (N-terminal) NeuroMab Cat#: 75-075, RRID: AB_2245198 Mouse monoclonal anti-b-Actin antibody Sigma-Aldrich Cat# A2228, RRID: AB_476697 Chicken polyclonal anti-GFP antibody Abcam Cat# ab13970, RRID: AB_300798 Mouse monoclonal ANTI-FLAG M2 antibody Sigma-Aldrich Cat# F1804, RRID: AB_262044 Guinea pig polyclonal anti-vGLUT1 antibody Synaptic System Antibodies Cat# 135304, RRID: AB_887878 Rabbit polyclonal anti-PMCA2 ATPase antibody Thermo Fisher Scientific Cat# PA1-915, RRID: AB_2243199 Goat polyclonal anti-CNTN1 antibody Abcam Cat# ab186067, RRID: AB_2747534 Rabbit monoclonal anti-b3-tubulin antibody Cell Signaling Technology Cat# 5568, RRID: AB_10694505 Rabbit polyclonal anti-EEA1 antibody Cell Signaling Technology Cat# 2411, RRID: AB_2096814 Rabbit monoclonal anti-PSD95 antibody Cell Signaling Technology Cat# 3450, RRID: AB_2292883 Rabbit polyclonal anti-CaMKII, phospho (Thr286) antibody Abcam Cat# ab32678, RRID: AB_725893 Rabbit polyclonal anti-Neuroligin 1 (extracellular) antibody Alomone Labs Cat# ANR-035, RRID: AB_2341006 Biological samples Pooled murine (CD1) CSF Bioreclamation IVT N/A Remnants of human CSF Bioreclamation IVT Cat# HUMANCSFR-0001211 CSF controls NIH Neurobiobank N/A CSF from people with ASD NIH Neurobiobank N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 Poly-D-lysine Sigma-Aldrich Cat# P0899 B27 Life Technologies Cat# 17504044 DL-APV Abcam Cat# ab120004 cOmplete, EDTA-free Protease inhibitor cocktail Sigma-Aldrich Cat# 11873580001 Phosphatase inhibitor cocktail 3 Sigma-Aldrich Cat# P0044 Trichloroacetic acid Sigma-Aldrich Cat# T8657 Picrotoxin Abcam Cat# ab120315 Strychnine Abcam Cat# ab120416 Glycine Sigma-Aldrich Cat# 50046 NBQX Tocris Cat# 1044 TAPI-0 Enzo Cat# BML-PI133-0001 GM6001 Sigma-Aldrich Cat# 364205 MMP9 inhibitor I Sigma-Aldrich Cat# 444278 Anti-FLAG M2 affinity gel MilliporeSigma Cat# A2220 Protein A/G agarose beads Thermo Fisher Cat# 20421 Fura-2, AM LifeTechnologies Cat# F1221 Adenosine 50-triphosphate (ATP) Sigma-Aldrich Cat# A2383 OptiMEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# MT10013CV CaptivA Protein A Affinity Resin RepliGen Cat# CA-PRI-0005 SR-101 Sigma-Aldrich Cat# S7635 Cal520, AM AAT Bioquest Cat# 21130 (Continued on next page) e1 Neuron 110, 627–643.e1–e9, February 16, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CNTNAP2 ELISA Kit RayBiotech Cat# ELH-Caspr2 Experimental models: Cell lines HEK293T ATCC CRL-11268 RRID: SCR_013869 Experimental models: Organisms/strains CD1 mice Charles River Laboratories Cat# CRL-22, RRID: IMSR_CRL:22 Cntnap2 KO mice Elior Peles, (Poliak et al., 2003) N/A Sprague Dawley male rats Charles River Laboratories Cat# Crl:SD, RRID: RGD_737891 C57BL6J mice Jackson Laboratories Cat# JAX:000664, RRID: IMSR_JAX:000664 Oligonucleotides CNTNAP2_GT_WT_F Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_WT_R Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_Neo_R Peter Penzes, (Gao et al., 2018) N/A Recombinant DNA FLAF-CNTNAP2 Peter Penzes, (Gao et al., 2018) N/A PMCA2w/b Gift from Emanuel Strehler Addgene plasmid #47586, RRID: Addgene_47586 pGP-CMV-GCAMP6s Gift from Douglas Kim Addgene plasmid # 40753 RRID: Addgene_40753 CNTN4.2-FC-His Gift from Woj Wojtowicz Addgene plasmid #72068, RRID: Addgene_72068 AAV1.Syn.GCaMP6f.WPRE.SV40 Gift from Douglas Kim Addgene plasmid #100837, RRID: Addgene_100837 pEGFP-N1 Clontech N/A, discontinued pDsRedExpress2-N1 Clontech Cat# 632537 shPMCA2 VectorBuilder VB171006-1032eez scramble shRNA VectorBuilder VB171006-1034vfh Fc-CNTNAP228-1261 Davide Comoletti, (Rubio-Marrero et al., 2016) N/A Software and algorithms FIJI (ImageJ) NIH https://imagej.net/, RRID: SCR_002285 NIS-Elements Nikon https://www.nikon.com/products/microscope- solutions/support/index.htm, RRID: SCR_014329 GraphPad Prism GraphPad Software Inc https://www.graphpad.com/scientific- software/prism, RRID: SCR_002798 ImageLab Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software, RRID: SCR_014210 IMARIS viewer Oxford instruments https://imaris.oxinst.com/, RRID: SCR_007370 MATLAB MathWorks https://www.mathworks.com/products/ matlab.html, RRID: SCR_001622 DAVID Bioinformatics Resources v6.8 Laboratory of Human Retrovirology and Immunoinformatics (LHRI) https://david.ncifcrf.gov/, RRID: SCR_001881 STRING v10.5 STRING Consortium https://string-db.org/, RRID: SCR_005223 Other Amicon Ultra 50 KDa 0.5 mL centrifugal filter devices MilliporeSigma Cat# UFC510096 Amicon Ultra-50 KDa 15 mL centrifugal filter devices MilliporeSigma Cat# UFC905024 50 kDa MWCO PES 50 mL centrifugal concentrator Sigma Aldrich Cat# GE28-9323-62 Neuron 110, 627–643.e1–e9, February 16, 2022 e2 ..

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS ..

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Article Title: Shed CNTNAP2 ectodomain is detectable in CSF and regulates Ca 2+ homeostasis and network synchrony via PMCA2/ATP2B2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CNTNAP2 antibody (C-term) Millipore Cat# AB5886, RRID: AB_92118 Mouse monoclonal anti-CNTNAP2 antibody (N-terminal) NeuroMab Cat#: 75-075, RRID: AB_2245198 Mouse monoclonal anti-b-Actin antibody Sigma-Aldrich Cat# A2228, RRID: AB_476697 Chicken polyclonal anti-GFP antibody Abcam Cat# ab13970, RRID: AB_300798 Mouse monoclonal ANTI-FLAG M2 antibody Sigma-Aldrich Cat# F1804, RRID: AB_262044 Guinea pig polyclonal anti-vGLUT1 antibody Synaptic System Antibodies Cat# 135304, RRID: AB_887878 Rabbit polyclonal anti-PMCA2 ATPase antibody Thermo Fisher Scientific Cat# PA1-915, RRID: AB_2243199 Goat polyclonal anti-CNTN1 antibody Abcam Cat# ab186067, RRID: AB_2747534 Rabbit monoclonal anti-b3-tubulin antibody Cell Signaling Technology Cat# 5568, RRID: AB_10694505 Rabbit polyclonal anti-EEA1 antibody Cell Signaling Technology Cat# 2411, RRID: AB_2096814 Rabbit monoclonal anti-PSD95 antibody Cell Signaling Technology Cat# 3450, RRID: AB_2292883 Rabbit polyclonal anti-CaMKII, phospho (Thr286) antibody Abcam Cat# ab32678, RRID: AB_725893 Rabbit polyclonal anti-Neuroligin 1 (extracellular) antibody Alomone Labs Cat# ANR-035, RRID: AB_2341006 Biological samples Pooled murine (CD1) CSF Bioreclamation IVT N/A Remnants of human CSF Bioreclamation IVT Cat# HUMANCSFR-0001211 CSF controls NIH Neurobiobank N/A CSF from people with ASD NIH Neurobiobank N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 Poly-D-lysine Sigma-Aldrich Cat# P0899 B27 Life Technologies Cat# 17504044 DL-APV Abcam Cat# ab120004 cOmplete, EDTA-free Protease inhibitor cocktail Sigma-Aldrich Cat# 11873580001 Phosphatase inhibitor cocktail 3 Sigma-Aldrich Cat# P0044 Trichloroacetic acid Sigma-Aldrich Cat# T8657 Picrotoxin Abcam Cat# ab120315 Strychnine Abcam Cat# ab120416 Glycine Sigma-Aldrich Cat# 50046 NBQX Tocris Cat# 1044 TAPI-0 Enzo Cat# BML-PI133-0001 GM6001 Sigma-Aldrich Cat# 364205 MMP9 inhibitor I Sigma-Aldrich Cat# 444278 Anti-FLAG M2 affinity gel MilliporeSigma Cat# A2220 Protein A/G agarose beads Thermo Fisher Cat# 20421 Fura-2, AM LifeTechnologies Cat# F1221 Adenosine 50-triphosphate (ATP) Sigma-Aldrich Cat# A2383 OptiMEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# MT10013CV CaptivA Protein A Affinity Resin RepliGen Cat# CA-PRI-0005 SR-101 Sigma-Aldrich Cat# S7635 Cal520, AM AAT Bioquest Cat# 21130 (Continued on next page) e1 Neuron 110, 627–643.e1–e9, February 16, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CNTNAP2 ELISA Kit RayBiotech Cat# ELH-Caspr2 Experimental models: Cell lines HEK293T ATCC CRL-11268 RRID: SCR_013869 Experimental models: Organisms/strains CD1 mice Charles River Laboratories Cat# CRL-22, RRID: IMSR_CRL:22 Cntnap2 KO mice Elior Peles, (Poliak et al., 2003) N/A Sprague Dawley male rats Charles River Laboratories Cat# Crl:SD, RRID: RGD_737891 C57BL6J mice Jackson Laboratories Cat# JAX:000664, RRID: IMSR_JAX:000664 Oligonucleotides CNTNAP2_GT_WT_F Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_WT_R Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_Neo_R Peter Penzes, (Gao et al., 2018) N/A Recombinant DNA FLAF-CNTNAP2 Peter Penzes, (Gao et al., 2018) N/A PMCA2w/b Gift from Emanuel Strehler Addgene plasmid #47586, RRID: Addgene_47586 pGP-CMV-GCAMP6s Gift from Douglas Kim Addgene plasmid # 40753 RRID: Addgene_40753 CNTN4.2-FC-His Gift from Woj Wojtowicz Addgene plasmid #72068, RRID: Addgene_72068 AAV1.Syn.GCaMP6f.WPRE.SV40 Gift from Douglas Kim Addgene plasmid #100837, RRID: Addgene_100837 pEGFP-N1 Clontech N/A, discontinued pDsRedExpress2-N1 Clontech Cat# 632537 shPMCA2 VectorBuilder VB171006-1032eez scramble shRNA VectorBuilder VB171006-1034vfh Fc-CNTNAP228-1261 Davide Comoletti, (Rubio-Marrero et al., 2016) N/A Software and algorithms FIJI (ImageJ) NIH https://imagej.net/, RRID: SCR_002285 NIS-Elements Nikon https://www.nikon.com/products/microscope- solutions/support/index.htm, RRID: SCR_014329 GraphPad Prism GraphPad Software Inc https://www.graphpad.com/scientific- software/prism, RRID: SCR_002798 ImageLab Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software, RRID: SCR_014210 IMARIS viewer Oxford instruments https://imaris.oxinst.com/, RRID: SCR_007370 MATLAB MathWorks https://www.mathworks.com/products/ matlab.html, RRID: SCR_001622 DAVID Bioinformatics Resources v6.8 Laboratory of Human Retrovirology and Immunoinformatics (LHRI) https://david.ncifcrf.gov/, RRID: SCR_001881 STRING v10.5 STRING Consortium https://string-db.org/, RRID: SCR_005223 Other Amicon Ultra 50 KDa 0.5 mL centrifugal filter devices MilliporeSigma Cat# UFC510096 Amicon Ultra-50 KDa 15 mL centrifugal filter devices MilliporeSigma Cat# UFC905024 50 kDa MWCO PES 50 mL centrifugal concentrator Sigma Aldrich Cat# GE28-9323-62 Neuron 110, 627–643.e1–e9, February 16, 2022 e2 ..

    Clinical Proteomics:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Multiplex sample analysis:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Plasmid Preparation:

    Article Title: Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model.
    Article Snippet: Article Soluble α2δ-1, altered in disease CSF, modulates network homeostasis and rescues deficits in a neuropsychiatric mouse model

    Article Title: Shed CNTNAP2 ectodomain is detectable in CSF and regulates Ca 2+ homeostasis and network synchrony via PMCA2/ATP2B2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CNTNAP2 antibody (C-term) Millipore Cat# AB5886, RRID: AB_92118 Mouse monoclonal anti-CNTNAP2 antibody (N-terminal) NeuroMab Cat#: 75-075, RRID: AB_2245198 Mouse monoclonal anti-b-Actin antibody Sigma-Aldrich Cat# A2228, RRID: AB_476697 Chicken polyclonal anti-GFP antibody Abcam Cat# ab13970, RRID: AB_300798 Mouse monoclonal ANTI-FLAG M2 antibody Sigma-Aldrich Cat# F1804, RRID: AB_262044 Guinea pig polyclonal anti-vGLUT1 antibody Synaptic System Antibodies Cat# 135304, RRID: AB_887878 Rabbit polyclonal anti-PMCA2 ATPase antibody Thermo Fisher Scientific Cat# PA1-915, RRID: AB_2243199 Goat polyclonal anti-CNTN1 antibody Abcam Cat# ab186067, RRID: AB_2747534 Rabbit monoclonal anti-b3-tubulin antibody Cell Signaling Technology Cat# 5568, RRID: AB_10694505 Rabbit polyclonal anti-EEA1 antibody Cell Signaling Technology Cat# 2411, RRID: AB_2096814 Rabbit monoclonal anti-PSD95 antibody Cell Signaling Technology Cat# 3450, RRID: AB_2292883 Rabbit polyclonal anti-CaMKII, phospho (Thr286) antibody Abcam Cat# ab32678, RRID: AB_725893 Rabbit polyclonal anti-Neuroligin 1 (extracellular) antibody Alomone Labs Cat# ANR-035, RRID: AB_2341006 Biological samples Pooled murine (CD1) CSF Bioreclamation IVT N/A Remnants of human CSF Bioreclamation IVT Cat# HUMANCSFR-0001211 CSF controls NIH Neurobiobank N/A CSF from people with ASD NIH Neurobiobank N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 Poly-D-lysine Sigma-Aldrich Cat# P0899 B27 Life Technologies Cat# 17504044 DL-APV Abcam Cat# ab120004 cOmplete, EDTA-free Protease inhibitor cocktail Sigma-Aldrich Cat# 11873580001 Phosphatase inhibitor cocktail 3 Sigma-Aldrich Cat# P0044 Trichloroacetic acid Sigma-Aldrich Cat# T8657 Picrotoxin Abcam Cat# ab120315 Strychnine Abcam Cat# ab120416 Glycine Sigma-Aldrich Cat# 50046 NBQX Tocris Cat# 1044 TAPI-0 Enzo Cat# BML-PI133-0001 GM6001 Sigma-Aldrich Cat# 364205 MMP9 inhibitor I Sigma-Aldrich Cat# 444278 Anti-FLAG M2 affinity gel MilliporeSigma Cat# A2220 Protein A/G agarose beads Thermo Fisher Cat# 20421 Fura-2, AM LifeTechnologies Cat# F1221 Adenosine 50-triphosphate (ATP) Sigma-Aldrich Cat# A2383 OptiMEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# MT10013CV CaptivA Protein A Affinity Resin RepliGen Cat# CA-PRI-0005 SR-101 Sigma-Aldrich Cat# S7635 Cal520, AM AAT Bioquest Cat# 21130 (Continued on next page) e1 Neuron 110, 627–643.e1–e9, February 16, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CNTNAP2 ELISA Kit RayBiotech Cat# ELH-Caspr2 Experimental models: Cell lines HEK293T ATCC CRL-11268 RRID: SCR_013869 Experimental models: Organisms/strains CD1 mice Charles River Laboratories Cat# CRL-22, RRID: IMSR_CRL:22 Cntnap2 KO mice Elior Peles, (Poliak et al., 2003) N/A Sprague Dawley male rats Charles River Laboratories Cat# Crl:SD, RRID: RGD_737891 C57BL6J mice Jackson Laboratories Cat# JAX:000664, RRID: IMSR_JAX:000664 Oligonucleotides CNTNAP2_GT_WT_F Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_WT_R Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_Neo_R Peter Penzes, (Gao et al., 2018) N/A Recombinant DNA FLAF-CNTNAP2 Peter Penzes, (Gao et al., 2018) N/A PMCA2w/b Gift from Emanuel Strehler Addgene plasmid #47586, RRID: Addgene_47586 pGP-CMV-GCAMP6s Gift from Douglas Kim Addgene plasmid # 40753 RRID: Addgene_40753 CNTN4.2-FC-His Gift from Woj Wojtowicz Addgene plasmid #72068, RRID: Addgene_72068 AAV1.Syn.GCaMP6f.WPRE.SV40 Gift from Douglas Kim Addgene plasmid #100837, RRID: Addgene_100837 pEGFP-N1 Clontech N/A, discontinued pDsRedExpress2-N1 Clontech Cat# 632537 shPMCA2 VectorBuilder VB171006-1032eez scramble shRNA VectorBuilder VB171006-1034vfh Fc-CNTNAP228-1261 Davide Comoletti, (Rubio-Marrero et al., 2016) N/A Software and algorithms FIJI (ImageJ) NIH https://imagej.net/, RRID: SCR_002285 NIS-Elements Nikon https://www.nikon.com/products/microscope- solutions/support/index.htm, RRID: SCR_014329 GraphPad Prism GraphPad Software Inc https://www.graphpad.com/scientific- software/prism, RRID: SCR_002798 ImageLab Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software, RRID: SCR_014210 IMARIS viewer Oxford instruments https://imaris.oxinst.com/, RRID: SCR_007370 MATLAB MathWorks https://www.mathworks.com/products/ matlab.html, RRID: SCR_001622 DAVID Bioinformatics Resources v6.8 Laboratory of Human Retrovirology and Immunoinformatics (LHRI) https://david.ncifcrf.gov/, RRID: SCR_001881 STRING v10.5 STRING Consortium https://string-db.org/, RRID: SCR_005223 Other Amicon Ultra 50 KDa 0.5 mL centrifugal filter devices MilliporeSigma Cat# UFC510096 Amicon Ultra-50 KDa 15 mL centrifugal filter devices MilliporeSigma Cat# UFC905024 50 kDa MWCO PES 50 mL centrifugal concentrator Sigma Aldrich Cat# GE28-9323-62 Neuron 110, 627–643.e1–e9, February 16, 2022 e2 ..

    shRNA:

    Article Title: Shed CNTNAP2 ectodomain is detectable in CSF and regulates Ca 2+ homeostasis and network synchrony via PMCA2/ATP2B2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CNTNAP2 antibody (C-term) Millipore Cat# AB5886, RRID: AB_92118 Mouse monoclonal anti-CNTNAP2 antibody (N-terminal) NeuroMab Cat#: 75-075, RRID: AB_2245198 Mouse monoclonal anti-b-Actin antibody Sigma-Aldrich Cat# A2228, RRID: AB_476697 Chicken polyclonal anti-GFP antibody Abcam Cat# ab13970, RRID: AB_300798 Mouse monoclonal ANTI-FLAG M2 antibody Sigma-Aldrich Cat# F1804, RRID: AB_262044 Guinea pig polyclonal anti-vGLUT1 antibody Synaptic System Antibodies Cat# 135304, RRID: AB_887878 Rabbit polyclonal anti-PMCA2 ATPase antibody Thermo Fisher Scientific Cat# PA1-915, RRID: AB_2243199 Goat polyclonal anti-CNTN1 antibody Abcam Cat# ab186067, RRID: AB_2747534 Rabbit monoclonal anti-b3-tubulin antibody Cell Signaling Technology Cat# 5568, RRID: AB_10694505 Rabbit polyclonal anti-EEA1 antibody Cell Signaling Technology Cat# 2411, RRID: AB_2096814 Rabbit monoclonal anti-PSD95 antibody Cell Signaling Technology Cat# 3450, RRID: AB_2292883 Rabbit polyclonal anti-CaMKII, phospho (Thr286) antibody Abcam Cat# ab32678, RRID: AB_725893 Rabbit polyclonal anti-Neuroligin 1 (extracellular) antibody Alomone Labs Cat# ANR-035, RRID: AB_2341006 Biological samples Pooled murine (CD1) CSF Bioreclamation IVT N/A Remnants of human CSF Bioreclamation IVT Cat# HUMANCSFR-0001211 CSF controls NIH Neurobiobank N/A CSF from people with ASD NIH Neurobiobank N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 Poly-D-lysine Sigma-Aldrich Cat# P0899 B27 Life Technologies Cat# 17504044 DL-APV Abcam Cat# ab120004 cOmplete, EDTA-free Protease inhibitor cocktail Sigma-Aldrich Cat# 11873580001 Phosphatase inhibitor cocktail 3 Sigma-Aldrich Cat# P0044 Trichloroacetic acid Sigma-Aldrich Cat# T8657 Picrotoxin Abcam Cat# ab120315 Strychnine Abcam Cat# ab120416 Glycine Sigma-Aldrich Cat# 50046 NBQX Tocris Cat# 1044 TAPI-0 Enzo Cat# BML-PI133-0001 GM6001 Sigma-Aldrich Cat# 364205 MMP9 inhibitor I Sigma-Aldrich Cat# 444278 Anti-FLAG M2 affinity gel MilliporeSigma Cat# A2220 Protein A/G agarose beads Thermo Fisher Cat# 20421 Fura-2, AM LifeTechnologies Cat# F1221 Adenosine 50-triphosphate (ATP) Sigma-Aldrich Cat# A2383 OptiMEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# MT10013CV CaptivA Protein A Affinity Resin RepliGen Cat# CA-PRI-0005 SR-101 Sigma-Aldrich Cat# S7635 Cal520, AM AAT Bioquest Cat# 21130 (Continued on next page) e1 Neuron 110, 627–643.e1–e9, February 16, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CNTNAP2 ELISA Kit RayBiotech Cat# ELH-Caspr2 Experimental models: Cell lines HEK293T ATCC CRL-11268 RRID: SCR_013869 Experimental models: Organisms/strains CD1 mice Charles River Laboratories Cat# CRL-22, RRID: IMSR_CRL:22 Cntnap2 KO mice Elior Peles, (Poliak et al., 2003) N/A Sprague Dawley male rats Charles River Laboratories Cat# Crl:SD, RRID: RGD_737891 C57BL6J mice Jackson Laboratories Cat# JAX:000664, RRID: IMSR_JAX:000664 Oligonucleotides CNTNAP2_GT_WT_F Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_WT_R Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_Neo_R Peter Penzes, (Gao et al., 2018) N/A Recombinant DNA FLAF-CNTNAP2 Peter Penzes, (Gao et al., 2018) N/A PMCA2w/b Gift from Emanuel Strehler Addgene plasmid #47586, RRID: Addgene_47586 pGP-CMV-GCAMP6s Gift from Douglas Kim Addgene plasmid # 40753 RRID: Addgene_40753 CNTN4.2-FC-His Gift from Woj Wojtowicz Addgene plasmid #72068, RRID: Addgene_72068 AAV1.Syn.GCaMP6f.WPRE.SV40 Gift from Douglas Kim Addgene plasmid #100837, RRID: Addgene_100837 pEGFP-N1 Clontech N/A, discontinued pDsRedExpress2-N1 Clontech Cat# 632537 shPMCA2 VectorBuilder VB171006-1032eez scramble shRNA VectorBuilder VB171006-1034vfh Fc-CNTNAP228-1261 Davide Comoletti, (Rubio-Marrero et al., 2016) N/A Software and algorithms FIJI (ImageJ) NIH https://imagej.net/, RRID: SCR_002285 NIS-Elements Nikon https://www.nikon.com/products/microscope- solutions/support/index.htm, RRID: SCR_014329 GraphPad Prism GraphPad Software Inc https://www.graphpad.com/scientific- software/prism, RRID: SCR_002798 ImageLab Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software, RRID: SCR_014210 IMARIS viewer Oxford instruments https://imaris.oxinst.com/, RRID: SCR_007370 MATLAB MathWorks https://www.mathworks.com/products/ matlab.html, RRID: SCR_001622 DAVID Bioinformatics Resources v6.8 Laboratory of Human Retrovirology and Immunoinformatics (LHRI) https://david.ncifcrf.gov/, RRID: SCR_001881 STRING v10.5 STRING Consortium https://string-db.org/, RRID: SCR_005223 Other Amicon Ultra 50 KDa 0.5 mL centrifugal filter devices MilliporeSigma Cat# UFC510096 Amicon Ultra-50 KDa 15 mL centrifugal filter devices MilliporeSigma Cat# UFC905024 50 kDa MWCO PES 50 mL centrifugal concentrator Sigma Aldrich Cat# GE28-9323-62 Neuron 110, 627–643.e1–e9, February 16, 2022 e2 ..

    Software:

    Article Title: Shed CNTNAP2 ectodomain is detectable in CSF and regulates Ca 2+ homeostasis and network synchrony via PMCA2/ATP2B2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CNTNAP2 antibody (C-term) Millipore Cat# AB5886, RRID: AB_92118 Mouse monoclonal anti-CNTNAP2 antibody (N-terminal) NeuroMab Cat#: 75-075, RRID: AB_2245198 Mouse monoclonal anti-b-Actin antibody Sigma-Aldrich Cat# A2228, RRID: AB_476697 Chicken polyclonal anti-GFP antibody Abcam Cat# ab13970, RRID: AB_300798 Mouse monoclonal ANTI-FLAG M2 antibody Sigma-Aldrich Cat# F1804, RRID: AB_262044 Guinea pig polyclonal anti-vGLUT1 antibody Synaptic System Antibodies Cat# 135304, RRID: AB_887878 Rabbit polyclonal anti-PMCA2 ATPase antibody Thermo Fisher Scientific Cat# PA1-915, RRID: AB_2243199 Goat polyclonal anti-CNTN1 antibody Abcam Cat# ab186067, RRID: AB_2747534 Rabbit monoclonal anti-b3-tubulin antibody Cell Signaling Technology Cat# 5568, RRID: AB_10694505 Rabbit polyclonal anti-EEA1 antibody Cell Signaling Technology Cat# 2411, RRID: AB_2096814 Rabbit monoclonal anti-PSD95 antibody Cell Signaling Technology Cat# 3450, RRID: AB_2292883 Rabbit polyclonal anti-CaMKII, phospho (Thr286) antibody Abcam Cat# ab32678, RRID: AB_725893 Rabbit polyclonal anti-Neuroligin 1 (extracellular) antibody Alomone Labs Cat# ANR-035, RRID: AB_2341006 Biological samples Pooled murine (CD1) CSF Bioreclamation IVT N/A Remnants of human CSF Bioreclamation IVT Cat# HUMANCSFR-0001211 CSF controls NIH Neurobiobank N/A CSF from people with ASD NIH Neurobiobank N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668019 Poly-D-lysine Sigma-Aldrich Cat# P0899 B27 Life Technologies Cat# 17504044 DL-APV Abcam Cat# ab120004 cOmplete, EDTA-free Protease inhibitor cocktail Sigma-Aldrich Cat# 11873580001 Phosphatase inhibitor cocktail 3 Sigma-Aldrich Cat# P0044 Trichloroacetic acid Sigma-Aldrich Cat# T8657 Picrotoxin Abcam Cat# ab120315 Strychnine Abcam Cat# ab120416 Glycine Sigma-Aldrich Cat# 50046 NBQX Tocris Cat# 1044 TAPI-0 Enzo Cat# BML-PI133-0001 GM6001 Sigma-Aldrich Cat# 364205 MMP9 inhibitor I Sigma-Aldrich Cat# 444278 Anti-FLAG M2 affinity gel MilliporeSigma Cat# A2220 Protein A/G agarose beads Thermo Fisher Cat# 20421 Fura-2, AM LifeTechnologies Cat# F1221 Adenosine 50-triphosphate (ATP) Sigma-Aldrich Cat# A2383 OptiMEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# MT10013CV CaptivA Protein A Affinity Resin RepliGen Cat# CA-PRI-0005 SR-101 Sigma-Aldrich Cat# S7635 Cal520, AM AAT Bioquest Cat# 21130 (Continued on next page) e1 Neuron 110, 627–643.e1–e9, February 16, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays CNTNAP2 ELISA Kit RayBiotech Cat# ELH-Caspr2 Experimental models: Cell lines HEK293T ATCC CRL-11268 RRID: SCR_013869 Experimental models: Organisms/strains CD1 mice Charles River Laboratories Cat# CRL-22, RRID: IMSR_CRL:22 Cntnap2 KO mice Elior Peles, (Poliak et al., 2003) N/A Sprague Dawley male rats Charles River Laboratories Cat# Crl:SD, RRID: RGD_737891 C57BL6J mice Jackson Laboratories Cat# JAX:000664, RRID: IMSR_JAX:000664 Oligonucleotides CNTNAP2_GT_WT_F Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_WT_R Peter Penzes, (Gao et al., 2018) N/A CNTNAP2_GT_Neo_R Peter Penzes, (Gao et al., 2018) N/A Recombinant DNA FLAF-CNTNAP2 Peter Penzes, (Gao et al., 2018) N/A PMCA2w/b Gift from Emanuel Strehler Addgene plasmid #47586, RRID: Addgene_47586 pGP-CMV-GCAMP6s Gift from Douglas Kim Addgene plasmid # 40753 RRID: Addgene_40753 CNTN4.2-FC-His Gift from Woj Wojtowicz Addgene plasmid #72068, RRID: Addgene_72068 AAV1.Syn.GCaMP6f.WPRE.SV40 Gift from Douglas Kim Addgene plasmid #100837, RRID: Addgene_100837 pEGFP-N1 Clontech N/A, discontinued pDsRedExpress2-N1 Clontech Cat# 632537 shPMCA2 VectorBuilder VB171006-1032eez scramble shRNA VectorBuilder VB171006-1034vfh Fc-CNTNAP228-1261 Davide Comoletti, (Rubio-Marrero et al., 2016) N/A Software and algorithms FIJI (ImageJ) NIH https://imagej.net/, RRID: SCR_002285 NIS-Elements Nikon https://www.nikon.com/products/microscope- solutions/support/index.htm, RRID: SCR_014329 GraphPad Prism GraphPad Software Inc https://www.graphpad.com/scientific- software/prism, RRID: SCR_002798 ImageLab Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software, RRID: SCR_014210 IMARIS viewer Oxford instruments https://imaris.oxinst.com/, RRID: SCR_007370 MATLAB MathWorks https://www.mathworks.com/products/ matlab.html, RRID: SCR_001622 DAVID Bioinformatics Resources v6.8 Laboratory of Human Retrovirology and Immunoinformatics (LHRI) https://david.ncifcrf.gov/, RRID: SCR_001881 STRING v10.5 STRING Consortium https://string-db.org/, RRID: SCR_005223 Other Amicon Ultra 50 KDa 0.5 mL centrifugal filter devices MilliporeSigma Cat# UFC510096 Amicon Ultra-50 KDa 15 mL centrifugal filter devices MilliporeSigma Cat# UFC905024 50 kDa MWCO PES 50 mL centrifugal concentrator Sigma Aldrich Cat# GE28-9323-62 Neuron 110, 627–643.e1–e9, February 16, 2022 e2 ..

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS ..

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software ..

    Control:

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS ..

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software ..

    Microscopy:

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS ..

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software ..

    Sequencing:

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software Article ll OPEN ACCESS ..

    Article Title: TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pEZ-3XHA-AnkG GeneCopoeia Cat# EX-Mm25668-M06 pEZ-3XFlag-Usp9X GeneCopoeia Cat#EX-Mm24322-M12 pRFP-C-RS Control Origene Cat#TR30015 pRFP-C-RS shUsp9X Origene Cat#TF510959 pGFP-C-RS shAnkG Smith et al., 2014 https://www.cell.com/neuron/abstract/ S0896-6273(14)00908-8 pmCherry-C1 Clontech Cat#632524 pEZ-3XHA-AnkG1-807 Yoon et al., 2020 HA-AnkG1-807 pEZ-3XFlag-Usp9X1555-1958 Yoon et al., 2020 Flag-Usp9XWt pEGFP-Usp9X1555-1958 Yoon et al., 2020 GFP-Usp9XWt pEZ-3XFlag-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 Flag-Usp9XS3A pEGFP-Usp9X1555-1958 S1593;1600;1609A Yoon et al., 2020 GFP-Usp9XS3A pMCSG53-6XHis-Usp9X1547-1962 Yoon et al., 2020 His-Usp9X1547-1962 pEZ-3XHA-AnkG480 This paper HA-AnkG480 Software and Algorithms FIJI (ImageJ) NIH https://fiji.sc/ NIS-Elements v4.51.00 Nikon https://www.nikon.com/products/ microscope-solutions/support/index.htm Prism 8 GraphPad https://www.graphpad.com/scientific- software/prism/ Maestro multiple sequence viewer Schrödinger https://www.schrodinger.com/ Maestro Bioluminate Schrödinger https://www.schrodinger.com/ PyMOL V1.8.2.0 Schrödinger https://pymol.org/2/ Image Lab 3.0 Bio-Rad Laboratories https://www.bio-rad.com/en-us/product/ image-lab-software ..



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